It is a high-yield transient expression kit of suspended human embryonic kidney cells (HKE 293 cells), which includes transient medium, feed medium, transfection reagent and buffer. The target protein is efficiently expressed by chemical transient transfection technology, and the efficient transient expression of suspended HKE 293 cells is realized. Structure analysis and drug development to provide a reliable guarantee.
TWayneLVPro™ HEK 293 cells were inoculated into LeExpro Medium, domestic medium A, domestic medium B, and imported medium C for Batch culture. The results showed that the highest cell density of LeExpro Medium was 1.07x107 cells/mL, and the cell growth performance was better than other media.
WayneLVPro™ HEK 293 cells were transferred to LeExpro Medium, domestic medium 2 and domestic medium 1 of the original process, and the supplement was the corresponding commercial supplement. High expression and low expression plasmid were selected, and after transfection, the expression levels were harvested for 5 days after culture. The results showed that the protein expression of LeExpro Medium was improved in different degrees in 7 items compared with other medium.
| Cat No. | Product name | Specification | Description |
|---|---|---|---|
| A24502 | Wayne293® Protein Transfection Kit | For 1L Culture | Including tranfection medium and feed medium, transfection reagents,transfection Buffer |
| A21506 | Wayne293® LeExpro Medium | 1000mL | Transfection Medium |
| A21902-01 | Wayne293® LeExpro Feed Medium | 100mL | Feed Medium |
| B31505 | QuaCell® Transfection Reagents | 5mL | Transfection Reagents |
| B31502-01 | QuaCell® Transfection Buffer | 100mL | Transfection Reagents |
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