QuaCell® Ready to Use Antifoam is designed for cell culture systems to quickly and efficiently meet the defoaming and antifoaming needs of bioprocesses. The product is treated by irradiation sterilization and can be directly used in sterile production environment. Its composition is clear and animal component-free, which is suitable for clinical sample preparation and large-scale commercial production of various types of biological drugs such as antibodies and fusion proteins.
Compared with the four ready to use antifoam, QuaCell® Ready to Use Antifoam (QuaCell Cat.# P11001-05), Antifoam 1 (Import Supplier 1), Antifoam 2 (Import supplier 2), Antifoam 3 (imported supplier 3) was selected with 5 different CHO cell lines from different sources, including CHO-K1, CHO-S and CHO-K1Q (QuaCell), and the expressed product was monoclonal antibody.
In terms of expression level, compared with the control group, different concentrations of antifoamers (120ppm/L and 360ppm/L) did not have significant effect on protein expression, and the protein expression of different cell lines showed relatively stable fluctuations in QuaCell antifoam, Antifoam1 and Antifoam3. There was no effect on protein expression with the increase of supplemental level. However, for Antifoma2, the expression level under 360ppm/L was lower in 5 different cell lines than that under 120ppm/L addition level.
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