Technical Data
- Excellent freeze recovery, high MSC recovery viability (>95%), unaffected cell phenotype
Case Study 1: MSC cells were treated with QuaCell® Powerful Cryopreservation medium, regular DMSO cryoprotectant, and a DMSO-free cryoprotectant. After the resuscitation test, MSC survived at 96%, 96%, and 95.5%, respectively. There were no significant differences between the three frozen stock solutions (P> 0.05). The MS surface markers (CD44, CD73, CD90, and CD105) were expressed by more than 95% of the cultivated cells, indicating that freezing MSC with this product had no effect on their immunophenotype.
Case Study 2: Cells with cryopreservation densities ranging from 0.3×106-30×106 cells/mL were treated independently using QuaCell® Powerful Cryopreservation Medium. The results indicated that the product performed better at densities ranging from 0.3×106cells/mL to 30×106 cells/mL.
