Procedure
Application
* This product supports high density cryopreservation of CHO-K1 cells.
QuaCell® Cryopreservation Medium
QuaCell® Cryopreservation Medium is a complete cell cryopreservation medium based on a optimized formulation，which has been validated by QuaCell technology team members over the years. The unique formulation is serum-free,animal component-free and chemically defined. With compound cryoprotectant containing DMSO, the cryopreservation medium avoids the formation of ice crystals by water molecules inside cells, and reduce the number of cations entering cells by forming protective layers outside cells. The double protection of intracellular and extracellular can greatly improve the recovery rate of cells. It is suitable for long-term cryopreservation of various animal cells.
Description
Technical Data
Technical Data
Comparison of QuaCell® Cryopreservation Solution of Various Cell Lines with Traditional Methods and Other Commercial Products.
Mapping detection cell line: Vero; QuaCell® serum-free cryopreservation medium VS control (80% DMEM+10% FBS+10% DMSO); right image detection cell line: SP2/0; QuaCell® serum-free cell cryopreservation medium VS Two commercial cryopreservation solutions and the control (80%DMEM+10%FBS+10%DMSO)
Freezing density: 5x10^6 cells/mL; after freezing at -80℃ for 3 days, move to liquid nitrogen for storage at regular intervals Take out a resuscitation and test the survival rate.
The results show that as the storage time increases, QuaCell® has a higher resuscitation rate than the control. Viability detection method: Countstar
QuaCell® Serum-Free Cell Cryopreservation Solution High-density cryopreservation CHO-K1Q test. Test cell line: a CHO-K1Q cell expressing Human IgG; freezing density: 1x10^8 cells/mL; frozen at -80°C for 3 days, transferred to liquid nitrogen for 89 days, resuscitation, continuous passage and detection of expression . The results showed that the proliferation and expression ability of CHO-K1Q cryopreserved in QuaCell® serum-free cell cryopreservation fluid had no effect.
Composition
Composition
| L-glutamine | Glucose | Hypoxanthine & Thymidine | Phenol red | Sodium bicarbonate | Hydrolysate |
|---|---|---|---|---|---|
| No | 6.0 g/L | No | No | Yes | No |
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